Iranian Journal of Materials Science and Engineering
Iran University of Science & Technology
Sun, Dec 15, 2024
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Volume 21, Issue 3 (September 2024)
IJMSE 2024, 21(3): 1-10 |
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Kotakonda M, V.S S, G A, Pakkiyan S N, Alungal N A, K M K, et al et al et al et al . Biogenic Synthesis of Papain Conjugated Copper Metallic Nanoparticles and Its Antibacterial and Antifungal Activity. IJMSE 2024; 21 (3) :1-10
URL: http://ijmse.iust.ac.ir/article-1-3547-en.html
URL: http://ijmse.iust.ac.ir/article-1-3547-en.html
Muddukrishnaiah Kotakonda 
, Sajisha V.S , Aiswarya G , Safeela Nasrin Pakkiyan , Najamol A Alungal , Mayoora Kiliyankandi K , Divya Thekke Kareth , Naheeda Ashraf Verali Parambil , Saranya Sasi Mohan , Renjini Anil Sheeba , Sarika Puthiya Veettil , Dhanish Joseph , Nishad Kakkattummal , Afsal Bin Haleem MP , Safeera Mayyeri , Thasneem Chemban Koyilott , Nasiya Nalakath , Samuel Thavamani B , Famila Rani J , Aruna Periyasamy , Chellappa V Rajesh , Rameswari Shanmugam , Marimuthu Poornima , Tina Raju , Roshni E R , Sirajudheen Mukriyan Kallungal , Lekshmi MS Panicker , Saranya K G , Shilpa V P
, Sajisha V.S , Aiswarya G , Safeela Nasrin Pakkiyan , Najamol A Alungal , Mayoora Kiliyankandi K , Divya Thekke Kareth , Naheeda Ashraf Verali Parambil , Saranya Sasi Mohan , Renjini Anil Sheeba , Sarika Puthiya Veettil , Dhanish Joseph , Nishad Kakkattummal , Afsal Bin Haleem MP , Safeera Mayyeri , Thasneem Chemban Koyilott , Nasiya Nalakath , Samuel Thavamani B , Famila Rani J , Aruna Periyasamy , Chellappa V Rajesh , Rameswari Shanmugam , Marimuthu Poornima , Tina Raju , Roshni E R , Sirajudheen Mukriyan Kallungal , Lekshmi MS Panicker , Saranya K G , Shilpa V P
Abstract: (10048 Views)
Biogenic synthesis of papain-conjugated copper metallic Nanoparticles and their antibacterial and antifungal activities Papain metallic conjugated nanoparticles (Papain-CuNPs) were synthesised using Papain and CuSO4.5H2O. Papain-CuNPs were characterized using UV-visible spectroscopy, FT-IR, HR-TEM, XRD, FE-SEM, zeta potential, and a zeta sizer. The antibacterial activity of papain-CuNPs against human infectious microorganisms (Citrobacter spp, Pseudomonas aeruginosa and Candida albicans) was investigated. The mechanism of action of papain-CuNPs was evaluated using FE-SEM and HRTM. UV spectroscopy confirmed the plasma resonance (SPR) at 679 nm, which indicated the formation of papain-CuNPs. The FT-IR spectrum absorbance peaks at 3927, 3865, 3842, 3363, 2978, and 2900 cm-1 indicate the presence of O-H and N-H of the secondary amine, and peaks at 1643 and 1572 cm-1 represent C=O functional groups in Papain-CuNPs. EDAX analysis confirmed the presence of copper in the papain-CuNPs. The zeta potential (-42.6 mV) and zeta size (99.66 d. nm) confirmed the stability and size of the nanoparticles. XRD confirmed the crystalline nature of the papain-CuNPs. FE-SEM and HRTM showed an oval structure, and the nano particles' 16.71244–34.84793 nm. The synthesized papain-NPs showed significant antibacterial activity against clinical P. aeruginosa (15 mm). MIC 125 µg/ml) showed bactericidal activity against P. aeruginosa and the mechanism of action of Papain-NPs was confirmed using an electron microscope by observing cell damage and cell shrinking. Papain-CuNPs have significant antibacterial activity and are thus used in the treatment of P. aeruginosa infections
Type of Study: Research Paper |
Subject:
Biomaterials
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